Protein Identification

Our protein identification services mainly include Protein Identification (Gel Spot/Gel Band Identification) and Proteome Profiling. We are happy to work with you to determine the best qualitative proteomics strategy for your project¹.

Proteome Profiling

Proteome profiling is a cost effective, high value solution for monitoring hundreds-to-thousands of proteins simultaneously. We perform sample digestion using sequencing-grade trypsin or alternative proteolytic method, and then trypsin-digested peptide samples are analyzed using nano-flow LC-MS/MS. The spectral counting method provides information on protein relative abundances². Besides, our HPLC sample fractionation systems are highly recommended for complex sample types to reduce sample complexity and maximize protein sequence coverage and detection dynamic range³.

Reference

[1] Baldwin MA. Protein identification by mass spectrometry: issues to be considered. Mol Cell Proteomics. 2004 Jan;3(1):1-9. Epub 2003 Nov 6. Review. PubMed PMID: 14608001. 

[2] Zhang, Y., et al., Tissue-based proteogenomics reveals that human testis endows plentiful missing proteins. J Proteome Res. 2015 Sep 4;14(9):3583-94.  

[3] Chandramouli K, Qian PY. Proteomics: challenges, techniques and possibilities to overcome biological sample complexity. Hum Genomics Proteomics. 2009 Dec 8;2009:239204.