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Single Cell Sequencing
Single cell sequencing is used for the study of heterogeneous samples, rare cell types, cell lineage relationships, and disease evolution. We offer high quality, affordable single cell services, with world-class support from our highly experienced single cell project experts.
Single Cell Sequencing Introduction
Single cell sequencing is commonly applied to solve problems associated with the study of heterogeneous samples, rare cell types, cell lineage relationships, and disease evolution. By using deep sequencing of DNA and RNA from a single cell, cellular functions of individual cells can be investigated in great detail.
BGI Genomics applies the superior method of Multiple Displacement Amplification (MDA) for single cell genomic DNA amplification (WGS/WES), and Switching Mechanism at 5′ End of RNA Template (SMART-Seq2) for single cell RNA amplification (transcriptome/RNA quantification). Contact us with your project requirements, our experts are here to help.
Experience of 1000s of single cell projects, our experts are here to ensure your success.
Guaranteed data quality at competitive pricing enabled by proprietary technology.
Rapid turnaround times and expert support, no project too big or small
Single Cell Sequencing-Service Overview
Sample Requirements
DNA
Single Cell: 1-2 cells - 4 µL PBS buffer
A Few Cells: 2-1000 cells - 4 µL PBS buffer
Amplified single cell gDNA/cDNA:
Concentration: >20 ng/µL, Quantity: >3 μg
RNA
Single Cell: 1-2 cells - 4 µL lysis buffer
A Few Cells: 2-1000 cells - 4 µL lysis buffer
Amplified single cell gDNA/cDNA:
Concentration: >0.2 ng/µL, Quantity: >1 ng
Total RNA for low input library:
Concentration: >50 pg/µL, Quantity: >2 ng;
RNA 28S/18S ≥1，RIN ≥7
Sequencing Standards
PE100 or PE150 sequencing option, depending on your application.
Guaranteed ≥85% of bases with a quality score of ≥Q30.
Cell sorting by mouth-controlled microcapillary pipetting system.
Key Service Details
• Typical 40 working days from sample QC acceptance to data delivery
• Expedited services are available, contact your local BGI Genomics specialist for details.
• Reports and output data files are delivered in industry standard file formats: BAM, .xls, .png and FASTQ data.
DNBSEQ™ Technology Platforms
BGI Genomics' Single Cell Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the competitive price in the industry. DNBSEQ™ can offer advantages in terms of lower amplification error rates and much lower duplication rates for some sequencing applications. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.
Contact us with any general enquiries or questions for our sequencing experts, we are here to help.
Access Further Sequencing Resources
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results. Access more sequencing resources below for full access to our range of educational resources and sample guides.
