Whole Genome Bisulfite Sequencing (WGBS)
Whole Genome Bisulfite Sequencing (WGBS) provides a scalable solution for cell characterization and gene expression profiling of hundreds to millions of cells. Tell us about your project, our experts are here to help.
Whole Genome Bisulfite Sequencing Introduction
Methylation of DNA at the fifth position in cytosine (5-mC) is a stable epigenetic modification and plays an important role in many biological processes, including gene silencing, suppression of transposable elements, genomic imprinting and X chromosome inactivation. Detection and quantification of methylation are critical to understand gene expression and other processes subjected to epigenetic regulation.
Whole genome bisulfite sequencing (WGBS) is used to detect methylated cytosines by treating the DNA with sodium bisulfite before sequencing. WGBS has become the gold standard for studying genome-wide methylation at single base resolution.
Cancer: Breast cancer, Bladder cancer, Lymph cancer Liver cancer
Complex Diseases: Autoimmune, Cardiovascular, Mental Disorders, Genetic Imprinting
Other Apps: Cell Differentiation, Embryonic Development, Methylation Mapping
Whole Genome Bisulfite Sequencing (WGBS)-BGI Service Overview
Sample Requirements
• Mass:≥0.5 μg
• Concentration:≥12.5 ng/μL
• Volume：15 μL
Sequencing Standards
• Guaranteed ≥90% of clean bases with quality score of Q20
• Guaranteed ≥99% bisulfite conversion rate
• Standard sequencing coverage ≥30X is recommended
Key Service Details
• Typical 26 working days from sample QC acceptance to filtered raw data availability
Whole Genome Bisulfite Sequencing Technology
BGI Genomics' Whole Genome Bisulfite Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.
Contact us with any general enquiries or questions for our sequencing experts, we are here to help.
Access Further Sequencing Resources
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results. Access more sequencing resources below for full access to our range of educational resources and sample guides.
