High Throughput Single Cell RNA-Seq

Description
Specification
Resources

High Throughput Single Cell RNA-Seq Service Description

Cell populations are heterogeneous and unsynchronized in their characteristics. Traditional bulk RNA sequencing generates comprehensive information but does not provide insight into variable expression between cells. High throughput single cell RNA-Seq provides a scalable solution for cell characterization and gene expression profiling of hundreds to millions of cells. High throughput single cell RNA-Seq technology is able to handle a large quantity of single cells fast and accurately, providing research solutions across a wide variety of applications including cancer, neurology, and immunology.

DNBSEQ™ Technology Platforms

BGI Genomics' Single Cell Sequencing Services are typically executed with our proprietary DNBSEQ™ NGS technology platform, for great sequencing data at the competitive pricing in the industry.

DNBSEQ™ can offer advantages in terms of lower amplification error rates and much lower duplication rates for some sequencing applications. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.




The major applications of single cell sequencing include:

  • Immune cell research

  • Tumor microenvironment research

  • Neuron system development research

  • Embryonic development and stem cell differentiation research

Project Workflow

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
  • SINGLE CELL PREPARATION
    Sample QC
  • LIBRARY PREPARATION
    Library QC
  • LIBRARY CIRCULARISATION
    Sequencing QC
  • SEQUENCING
    Data QC
  • BIOINFORMATICS

Sequencing Service Specification

BGI Genomics' High throughput single cell RNA-Seq services are performed on our DNBSEQ™ technology platform:
  • Sequencing

    • Guaranteed ≥85% of bases with a quality score of ≥Q30
    • DNBSEQ™ PE100 or PE150 options
    • Raw data and bioinformatics analysis are available in standard file formats
  • Turnaround Time

    • Typical 40 working days from sample QC acceptance to data delivery
    • Expedited services are available, contact your local BGI Genomics specialist for details

Sample Requirements

  • Cell line/Tissue/PBMC:

    • After sample processing, freeze cells at a final concentration of 1x10^6 cells/mL in cryopreserved tubes (Cell freezing medium: 90% FBS+10% DMSO)

Data Analysis

In addition to raw data output, BGI offers a range of standard and customized bioinformatics pipelines for your single cell sequencing project.
STANDARD ANALYSIS FOR HIGH THROUGHPUT SINGLE CELL RNA-SEQ
  • Sequencing Result
  • Comparison Result
  • Quantitative Analysis
  • Data QC
  • Cell Cluster
  • Differential Gene Identification Between Samples
  • Cell Cluster Annotation
  • Marker Gene/Differentially Expressed Gene GO Pathway
  • Marker Gene/Differentially Expressed Gene KEGG Pathway Enrichment
  • RNA Velocity
  • CellPhone DB
  • Pseudotemporal Ordering
  • Customized Cell Cluster Analysis
  • Customized Cell Cluster Annotation by Database of your choice
  • Gene-Protein Interaction Analysis
  • Gene Function Enrichment Analysis
  • Dynamic Interactive Analysis of Cell Type and Cell Trajectory Analysis
  • Multi-Dimensional Analysis of Differential Gene Between Cell Types/Samples
CUSTOMIZED ANALYSIS

Further customization of bioinformatics analysis to suit your unique project is available: Please contact your BGI Genomics technical representative.

TOP