High Throughput Single Cell RNA-Seq

Description
Specification
Resources

High Throughput Single Cell RNA-Seq Service Description

Cell populations are heterogeneous and unsynchronized in their characteristics. Traditional bulk RNA sequencing generates comprehensive information but does not provide insight into variable expression between cells. High throughput single cell RNA-Seq provides a scalable solution for cell characterization and gene expression profiling of hundreds to millions of cells. High throughput single cell RNA-Seq technology is able to handle a large quantity of single cells fast and accurately, providing research solutions across a wide variety of applications including cancer, neurology, and immunology.

DNBSEQ™ NGS technology platform

BGI's Single Cell Sequencing service is typically executed with our proprietary DNBSEQ™ NGS technology platform, for great sequencing data at the competitive pricing in the industry.

DNBSEQ™ is a proprietary sequencing technology, developed by MGI. This system is powered by combinatorial Probe-Anchor Synthesis (cPAS), linear isothermal Rolling-Circle Replication and DNA Nanoballs (DNB) technology, followed by high-resolution digital imaging.



The major applications of single cell sequencing include:
  • Discovering new cells and cell subtypes

  • Studying tissue and cell group heterogeneity

  • Studying tumor-immune cell interaction

  • Searching for marker genes for drug development

Project Workflow

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
  • SINGLE CELL PREPARATION
    Sample QC
  • LIBRARY PREPARATION
    Library QC
  • LIBRARY CIRCULARISATION
    Sequencing QC
  • SEQUENCING
    Data QC
  • BIOINFORMATICS

Sequencing Service Specification

BGI's High throughput single cell RNA-Seq services are performed on our DNBSEQ™ technology platform:
  • Sequencing

    • Guaranteed ≥90% of bases with quality score of ≥Q20
    • DNBSEQ™ PE100 or PE150 options
    • Raw data and bioinformatics analysis are available in standard file formats
  • Turnaround Time

    • Typical 35 working days from sample QC acceptance to filtered raw data availability
    • Expedited services are available, contact your local BGI specialist for details

Sample Requirements

  • Cell line/Tissue/PBMC:

    • After sample processing, freeze cells at a final concentration of 1x10^6 cells/ml in cryopreserved tubes (Cell freezing medium: 90% FBS+10% DMSO)

Data Analysis

In addition to raw data output, BGI offers a range of standard and customized bioinformatics pipelines for your single cell sequencing project.
STANDARD ANALYSIS FOR HIGH THROUGHPUT SINGLE CELL RNA-SEQ
  • Sequencing Result

  • Comparison Result

  • Quantitative Analysis

  • Data QC

  • Cell Cluster

  • Differential Gene Identification Between Samples

  • Cell Cluster Annotation

  • Marker Gene/Differentially Expressed Gene GO Pathway

  • Marker Gene/Differentially Expressed Gene KEGG Pathway Fnrichment

  • RNA Velocity

  • CellPhone DB

  • Pseudotemporal Ordering

  • Customized Cell Cluster Analysis

  • Customized Cell Cluster Annotation By Database of your choice

  • Gene-Protein Interaction Analysis

  • Gene Function Enrichment Analysis

  • Dynamic Interactive Analysis of Cell Type And Cell Trajectory Analysis

  • Different Dimension Analysis of Differential Gene Between Cell Types/Samples

CUSTOMIZED ANALYSIS

Further customization of bioinformatics analysis to suit your unique project is available: Please contact your BGI technical representative

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