High-throughput targeted metabolomics HM Pro2300

DESCRIPTION
SPECIFICATION
RESOURCES

Service Description

BGI Genomics has extensive experience in the field of targeted metabolomics spanning accurate quantification of target metabolites, verification of hypothesis by untargeted metabolomics or other omics, and metabolic model study for specific metabolites1.

HM Pro2300

The HM Pro2300 panel uses LC-MS/MS technology for high-throughput targeted metabolomics detection. HM Pro2300 can simultaneously detect more than 2000 metabolites, including 700 small molecules and more than 1600 lipids which covers all kinds of important metabolites and core metabolic pathways. We also provide HM700 and HML1600, only detecting hydrophilic small molecules and lipids respectively.

Project Workflow

References

[1] Liu R., et al., Gut microbiome and serum metabolome alterations in obesity and after weight-loss intervention. Nat Med, 2017. 23(7): p. 859-868.

Mass Spectrometry Service Specification

HM Pro2300 high-throughput targeted metabolomics services are performed using Waters ACQUITY UPLC and SCIEX QTRAP 5500, 6500+.

  • Sample Preparation and Services

    • Custom-developed UPLC-MRM/MS methods combine isotope internal standard technology
    • Small molecules and lipids are extracted respectively to increase metabolome depth
    • Using SCIEX QTRAP 6500+, 5500 detect respectively small molecules and lipids to increase the scope of detection

  • Quality Standard

    • Summary includes all methods and data analysis
    • Reports provided in Excel or PDF format

  • Turn Around Time

    • Typical 6-8 weeks after sample receipt

Sample Requirements

We accept metabolite samples in a variety of formats. Contact your BGI Genomics account representative for details for your samples. Note: Cell samples need to be divided into two copies of the same
SAMPLE TYPERECOMMENDED SAMPLE AMOUNTMINIMUM SAMPLE AMOUNT
Serum, plasma, urine≥ 200 µL≥ 100 µL
Animal and clinical tissues, feces and intestinal contents, microorganism≥ 100 mg≥ 40 mg
Cell2×10^72×10^5
Culture medium, fermentation medium≥ 1 mL≥ 200 µL

Data Analysis

Data Analysis
  • Data analysis performed with metaX and BGI Genomics library
  • Data quality control
  • Standard curves
  • Quantification results

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