DESCRIPTION
SPECIFICATION
RESOURCES
Project Workflow
FFPE sample analysis when there are suitable library construction samples:
- FFPE samples
- Dewaxing and rehydration
- Decrosslink
- Protein
- Enzymolysis
- LC-MS/MS DIA analysis
- Data analysis
FFPE sample analysis when there are no suitable library construction samples:
- FFPE samples
- Dewaxing and rehydration
- Decrosslink
- Protein
- Enzymolysis
- LC-MS/MS DDA analysis
- Data analysis
References
[1] Marchione D M, et al., HYPERsol: High-Quality Data from Archival FFPE Tissue for Clinical Proteomics. J Proteome Res, 2020 Feb 7; 19(2): 973–983.
How to order
Mass Spectrometry Service Specification
FFPE Proteomics services are performed using nano-flow liquid chromatography and cutting-edge Q Exactive HF/HF-X mass spectrometer.
Sample Preparation and Services
- The QC analysis of enzymatic hydrolysis is strictly controlled by LC-MS
- DDA-Spectral Library generated using HPLC technology and sample fractionation
Quality Standard
- Summary includes all methods and data analysis
- Reports provided in Excel or PDF format, RAW files available upon request
Turn Around Time
- Typical 5-6 weeks from sample QC acceptance to data report delivery
Sample Requirements
DATA ANALYSIS
- Data analysis and validation performed with Mascot
- Protein identification and quantification list
- Differential proteins data statistics and volcano plot
- Principal component analysis (PCA) and cluster analysis
- Protein GO/COG/KOG/Pathway annotation
- GO/COG/KOG enrichment analysis of differential proteins
- Protein-protein interaction analysis
- Protein subcellular localization analysis
- Time series analysis
- Repeatability analysis
