BGI has extensive experience in the field of nanoproteomics spanning cellular heterogeneity research across rare cell populations, hard-to-obtain clinical specimens, and pathological tissues.

Service Description

Nanoproteomics refers to quantitative proteomics analysis of small cell populations (typically < 5,000 cells) by the combination of in-situ cleavage and DDA label free technology, and offers possibilities for analysis unavailable with conventional protein extraction and mass spectrometry1.

Nanoproteomics

<img src="https://cdn.it.bgi.com/source/nano workflow242.png" style="max-width:100%;" contenteditable="false"/> References [1] L. Yi, et al., Advances in microscale separations towards nanoproteomics applications, 2017.1523: 40–48.

Project Workflow

How to order

Nanoproteomics services are performed using nano-flow liquid chromatography and cutting-edge Orbitrap Fusion Lumos mass spectrometer.

<ul><li>The QC of enzymatic hydrolysis is strictly controlled by LC-MS</li><li>DDA-Spectral Library generated using HPLC technology and sample fractionation</li><li>2h/fraction; 2h for DDA analysis</li></ul>

Sample Preparation and Services

<ul><li>Summary includes all methods and data analysis</li><li>Reports provided in Excel or PDF format, RAW files available upon request</li></ul>

Quality Standard

<ul><li>Typical 5-6 weeks from sample QC acceptance to data report delivery</li></ul>

Turn Around Time

Mass Spectrometry Service Specification

Sample Requirements

<ul><li>Data analysis and validation performed with Mascot</li><li>Protein identification and quantification list</li><li>Cluster analysis of expression patterns across multiple samples</li><li>Protein GO/COG/KOG/Pathway annotation</li><li>GO/Pathway enrichment analysis of differential proteins</li><li>Protein-protein interaction analysis</li><li>Protein subcellular localization analysis</li><li>Repeatability analysis</li><li>Quantitative proteomics and phosphoproteomics correlation analysis</li></ul>