DESCRIPTION
SPECIFICATION
RESOURCES
Project Workflow
Gel Spot Identification
We can identify proteins present in gel spots.
- GEL SPOT
- DECOLORIZATION
- PROTEIN DIGESTION
- LC-MS/MS (DDA)
- PROTEIN IDENTIFICATION
Gel Band Identification
We can identify proteins present in gel bands or protein solution.
- GEL BAND OR PROTEIN SOLUTION
- DECOLORIZATION
- PROTEIN DIGESTION
- LC-MS/MS (DDA)
- BIOINFORMATICS ANALYSIS
How to order
Service Specification
Sample Preparation and Services
- Digestion performed using sequencing-grade trypsin
- 60 min nano-flow LC-MS/MS
Quality Standard
- Summary includes all methods and data analysis
- Reports provided in Excel or PDF format, RAW files available upon request
Turn Around Time
- Typical 2 weeks after sample receipt for raw data delivery
Sample Requirements
Projects that need enrichment for acetylation and ubiquitination studies is not accepted.
| PRODUCT SAMPLE | GEL BANDIDENTIFICATION | MODIFICATION SITE IDENTIFICATION |
|---|---|---|
| Coomassive or silver stained protein spot/band | Protein ≥ 1 μg | Protein ≥ 1μg. Projects that need enrichment is not accepted. |
| Single protein (protein solution purified after IP or CO-IP) | ≥ 5 μg,≥ 0.5 μg/μL | ≥ 10 μg, ≥ 0.5 μg/μL. If enrichment is required, the amount of sample sent needs to be increased by 3 times. |
Data Analysis
ADVANCED BIOINFORMATICS
- Data analysis and validation performed with Mascot
- GO (Gene ontology) category analysis
- COG (Cluster of Orthologous Groups of proteins) category analysis
- Pathway analysis
Resources
We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
