Cell populations are heterogeneous and unsynchronized in their characteristics. Traditional bulk RNA sequencing generates comprehensive information but does not provide insight into variable expression between cells.&nbsp;High throughput single cell RNA-Seq&nbsp;provides a scalable solution for cell characterization and gene expression profiling of hundreds to millions of cells.&nbsp;High throughput single cell RNA-Seq&nbsp;technology is able to handle a large quantity of single cells fast and accurately, providing research solutions across a wide variety of applications including cancer, neurology, and immunology.

High Throughput Single Cell RNA-Seq Service Description

BGI’s Single Cell Sequencing service is typically executed with our proprietary DNBSEQ™ NGS technology platform, for great sequencing data at the lowest cost in the industry.DNBSEQ™&nbsp;is a proprietary sequencing technology, developed by MGI.&nbsp;This system is powered by combinatorial Probe-Anchor Synthesis (cPAS), linear isothermal Rolling-Circle Replication and DNA Nanoballs (DNB™) technology, followed by high-resolution digital imaging. <img src="https://cdn.it.bgi.com/source/BGI Human Whole Genome Sequencing Brochure pornt-20220930-01.png" style="max-width:100%;" contenteditable="false"/> The major applications of single cell sequencing include:

DNBSEQ™ NGS technology platform

Discovering new cells and cell subtypes

Studying tissue and cell group heterogeneity

Studying tumor-immune cell interaction

Searching for marker genes for drug development

We care for your samples from the start through to the result reporting. Highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.

Project Workflow

How to order

BGI's High throughput single cell RNA-Seq services are performed on our DNBSEQ™ technology platform:

<ul><li>Guaranteed ≥90% of bases with quality score of ≥Q20</li><li>DNBSEQ™ PE100 or PE150 options</li><li>Raw data and bioinformatics analysis are available in standard file formats</li></ul>

<ul><li>Typical 35 working days from sample QC acceptance to filtered raw data availability</li><li>Expedited services are available, contact your local BGI specialist for details</li></ul>

Turnaround Time

<ul><li>After sample processing, freeze cells at a final concentration of 1x10^6 cells/ml in cryopreserved tubes (Cell freezing medium: 90% FBS+10% DMSO)</li></ul>

Cell line/Tissue/PBMC:

Sequencing Service Specification

<ul><li>Sequencing Result</li><li>Comparison Result</li><li>Quantitative Analysis</li><li>Data QC</li><li>Cell Cluster</li><li>Differential Gene Identification Between Samples</li><li>Cell Cluster Annotation</li><li>Marker Gene/Differentially Expressed Gene GO Pathway</li><li>Marker Gene/Differentially Expressed Gene KEGG Pathway Fnrichment</li><li>RNA Velocity</li><li>CellPhone DB</li><li>Pseudotemporal Ordering</li><li>Customized Cell Cluster Analysis</li><li>Customized Cell Cluster Annotation By Database of your choice</li><li>Gene-Protein Interaction Analysis</li><li>Gene Function Enrichment Analysis</li><li>Dynamic Interactive Analysis of Cell Type And Cell Trajectory Analysis</li><li>Different Dimension Analysis of Differential Gene Between Cell Types/Samples</li></ul>

STANDARD ANALYSIS FOR HIGH THROUGHPUT SINGLE CELL RNA-SEQ

In addition to raw data output, BGI offers a range of standard and customized bioinformatics pipelines for your single cell sequencing project.

Data Analysis

Further customization of bioinformatics analysis to suit your unique project is available: Please contact your BGI technical representative

CUSTOMIZED ANALYSIS

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