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        Sample Submission and Shipping Guidelines

        DNA Sample Requirement General Guidelines for:


        • HISEQ Platform Based Services
        • DNBSEQ™ Based Services
        • PACBIO Platform Based Services

        For questions on submitting other sample types or for custom sequencing solutions, please contact our Project Management teams or your BGI Sales Representative to discuss further.
        Contact Support: info@bgi.com

        For HISEQ Platform Based Services

        WHOLE GENOME RESEQUENCING ≤800BP NORMAL LIBRARY (HUMAN/PLANT/ANIMAL/FUNGAL/BACTERIAL/METAGENOMIC)

        • Sample type: genomic DNA/PCR product

        • Quality requirements: >1µg, >12.5ng/µl

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        DE NOVO GENOME SEQUENCING ≤800BP NORMAL LIBRARY (HUMAN/PLANT/ANIMAL/FUNGAL/BACTERIAL/METAGENOMIC)

        • Sample type: genomic DNA/PCR product

        • Quality requirements: >1.5µg, >20ng/µl

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        DE NOVO GENOME SEQUENCING 2KB≤X≤6KB (HUMAN/PLANT/ANIMAL/FUNGAL/BACTERIAL/METAGENOMIC)

        • Sample type: genomic DNA/PCR product

        • Quality requirements: m>20µg, c>110ng/µl, no degradation or slight degradation

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        DE NOVO GENOME SEQUENCING 8KB≤X≤10KB (HUMAN/PLANT/ANIMAL/FUNGAL/BACTERIAL/METAGENOMIC)

        • Sample type: genomic DNA

        • Quality requirements: m>30µg, c>110ng/µl, no degradation or slight degradation

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        DE NOVO GENOME SEQUENCING 20KB (HUMAN/PLANT/ANIMAL/FUNGAL/BACTERIAL/METAGENOMIC)

        • Sample type: genomic DNA

        • Quality requirements: m>40µg, c>110ng/µl, no degradation or slight degradation

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        DE NOVO GENOME SEQUENCING 40KB (HUMAN/PLANT/ANIMAL/FUNGAL/BACTERIAL/METAGENOMIC)

        • Sample type: genomic DNA

        • Quality requirements: m>50µg, c>110ng/µl, no degradation or slight degradation

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        DE NOVO GENOME SEQUENCING ≤800BP PCR FREE LIBRARY (HUMAN/PLANT/ANIMAL/FUNGAL/BACTERIAL/METAGENOMIC)

        • Sample type: genomic DNA

        • Quality requirements: m>10µg, c>30ng/µl, no degradation or slight degradation

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice.

        WHOLE EXOME SEQUENCING (HUMAN/ANIMAL): GENOMIC DNA

        • Quality requirements: m>1µg, c>12.5ng/µl, no degradation or slight degradation

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice.

        WHOLE EXOME SEQUENCING (HUMAN/ANIMAL): FFPE GENOMIC DNA

        • Quality requirements: m>1µg, c>12.5ng/µl, main fragment size ≥250bp

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        WHOLE EXOME SEQUENCING (PLANT)

        • Sample type: genomic DNA

        • Quality requirements: m>10µg, c>30ng/µl, no degradation or slight degradation

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        TARGET REGION SEQUENCING: GENOMIC DNA

        • Quality requirements: m>1µg, c>12.5ng/µl, no degradation or slight degradation

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        TARGET REGION SEQUENCING: FFPE GENOMIC DNA

        • Quality requirements: m>1µg, c>12.5ng/µl, main fragment size ≥250bp

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        CHIP SEQUENCING

        • Sample type: ChIP-ed DNA

        • Quality requirements: m>10ng, c>1ng/µl, Major Peak in 100bp-500bp (Agilent 2100）

        • Sample volume for QC: 1ul for Qubit, 2ul for Agilent 2100

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        RAD- SEQUENCING

        • Sample type: genomic DNA

        • Quality requirements: m>300ng, c>10ng/µl, no degradation or slight degradation, No RNA and Protein contamination

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        GENOTYPING BY SEQUENCING

        • Sample type: genomic DNA

        • Quality requirements: m>1µg, c>20ng/µl, no degradation or slight degradation, No RNA and Protein contamination

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        TARGET REGION BISULFITE SEQUENCING (TBS)

        • Sample type: genomic DNA

        • Quality requirements: m>3µg, c>37.5ng/µl, no degradation or slight degradation, No RNA and Protein contamination

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        WHOLE GENOME BISULFITE SEQUENCING

        • Sample type: genomic DNA

        • Quality requirements: m>1µg, c>15ng/µl, no degradation or slight degradation

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        META RDNA AMPLICON SEQUENCING

        • Sample type: genomic DNA

        • Quality requirements: m>1µg, c>20ng/µl, no degradation or slight degradation

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        For DNBSEQ sequencing technology Based Services

        CHIP SEQUENCING

        • Sample type: ChIP-ed DNA

        • Quality requirements: m>10ng, c>1ng/µl, Major Peak in 100bp-500bp (Agilent 2100）

        • Sample volume for QC: 1ul for Qubit, 2ul for Agilent 2100

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        WHOLE EXOME SEQUENCING

        • Sample type: genomic DNA

        • Quality requirements: m>1µg, c>12.5ng/µl, No degradation or slight degradation

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        WHOLE GENOME SEQUENCING

        • Sample type: genomic DNA

        • Quality requirements: m>1µg, c>12.5ng/µl, No degradation or slight degradation

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Re-suspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        For PACBIO Platform Based Services

        PACBIO DNA SEQUENCING (20KB)

        • Sample type: genomic DNA

        • Quality requirements: m>10.2ug, c>80ng/µl, no degradation or slight degradation

        • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        PACBIO DNA SEQUENCING (10KB）

        • Sample type: genomic DNA

        • Quality requirements: m>10.2µg, c>40ng/µl, No degradation or slight degradation

        • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        PACBIO DNA SEQUENCING (5KB）

        • Sample type: genomic DNA

        • Quality requirements: m>4.2µg, c>50ng/µl, No degradation or slight degradation

        • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        PACBIO DNA SEQUENCING (2KB)

        • Sample type: genomic DNA

        • Quality requirements: m>1.7µg, c>50ng/µl, No degradation or slight degradation

        • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        PACBIO DNA SEQUENCING (1KB)


        • Sample type: genomic DNA

        • Quality requirements: m>1.2µg, c>40ng/µl, no degradation or slight degradation

        • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice

        PACBIO DNA SEQUENCING (200BP/500BP)

        • Sample type: genomic DNA

        • Quality requirements: m>0.52µg, c>20ng/µl, no degradation or slight degradation

        • OD260/280:1.8-2.0, OD260/230:2.0-2.2, Qubit/OD>0.5

        • Sample volume for QC: 1ul for Qubit, 100ng for Electrophoresis

        • Resuspension Buffer: Nuclease-free water, TE buffer (<0.1mM EDTA), EB

        • Shipment: Dry ice