BGI has extensive experience in the field of untargeted metabolomics with developed reliable workflows using market leading technologies and a bioinformatics infrastructure that is second to none.

Service Description

By applying state-of-the-art LC-MS/MS systems and techniques, BGI offers validated workflows of untargeted metabolomics to support biomedical and biotechnology applications1. Our Untargeted Metabolomics services are designed to obtain a metabolite profile and screen for differentially-expressed molecules in the sample.

Untargeted Metabolomics

Project Workflow

<h4>[1]&nbsp;Liu R., et al., Gut microbiome and serum metabolome alterations in obesity and after weight-loss intervention. Nat Med, 2017. 23(7): p. 859-868.</h4>

References

How to order

Untargeted Metabolomics services are performed using Waters ACQUITY UPLC and cutting-edge mass spectrometer (Q Exactive HF/HF-X).

<ul><li>Increase metabolome depth of coverage using both ACQUITY UPLC BEH C18 and Amide column</li><li>(+) and (-) ESI detection by a Q Exactive HF/HF-X mass spectrometer</li></ul>

Sample Preparation and Services

<ul><li>Summary includes all methods and data analysis</li><li>Reports provided in Excel or PDF format, Raw files available upon request</li></ul>

Quality Standard

<ul><li>Typical 4-6 weeks after sample receipt for raw data delivery</li></ul>

Turn Around Time

Mass Spectrometry Service Specification

We accept metabolite samples in a variety of formats. Contact your BGI account representative for details for your samples.

Sample Requirements

<ul><li>Data analysis performed with metaX1 and BGI library</li><li>T-test, PCA, PLS-DA for differential metabolite identification</li><li>Cluster analysis</li><li>Metabolite pathway annotation&nbsp;</li><li>ROC analysis</li><li>Metabolite correlation analysis</li></ul>